Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 3, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/3/2/025002
Keywords
gamma globulin; gold nanoparticle; bio-conjugates; fluorescence spectroscopy
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Funding
- National Institute of Technology, Hamirpur (HP), India
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The interaction of Citrate-capped gold nanoparticles (AuNPs) with Bovine gamma globulin (BGG) was studied using Fourier transform infrared (FTIR), UV-Visible and Fluorescence spectroscopy. FTIR has confirmed the conjugation of AuNPs and BGG. Fluorescence quenching of tryptophan has confirmed the strong interaction between BGG and AuNPs. UV-Visible and Fluorescence spectroscopy have investigated the extent of interaction by determining the binding constants. Binding constants evaluated from UV-Visible and Fluorescence data are in good agreement with each other. An independent class of binding site on BGG for AuNPs has been predicted, where AuNPs interact with a highly solvent accessible tryptophan residue.
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