Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 3, Issue 4, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/3/4/042001
Keywords
bioorthogonal; fluorescent; bioimaging
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Funding
- Hungarian Academy of Sciences (Lendulet) [LP2013-55/2013]
- Hungarian Scientific Research Fund [NN-110214]
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This review ventures to summarize the latest developments in bioorthogonal fluorescent imaging labels with a special focus on bioimaging applications. We briefly summarize the most preferred means of bioorthogonal tagging schemes for the labeling of specific biomolecular structures. The review is structured by the type of the fluorescent labels that can address the problems that most commonly compromise fluorescent imaging techniques, i.e. the autofluorescence of biomolecules, the background fluorescence of unreacted reagents, and photobleaching. Thus, we present (i) farred/near-infra-red emitting dyes, (ii) fluorogenic scaffolds, and (iii) nanoparticle-based signaling platforms.
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