Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 139, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/57640
Keywords
Immunology and Infection; Issue 139; Dendritic Cell; Micelles; Hybrid Materials; DNA Nanotechnology; Animal Administration; Murine Lymph Nodes; Adjuvants
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Funding
- Creative Materials Discovery Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [NRF-2017M3D1A1039421]
- Marine Biotechnology Program - Ministry of Oceans and Fisheries, Republic of Korea [20150220]
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For evaluation of a new therapeutic agent for immunotherapy or vaccination, analysis of immune cell activation in lymphatic tissues is essential. Here, we investigated immunological effects of a novel lipid-DNA immunostimulant in nanoparticle form from different administration routes in the mouse: oral, intranasal, subcutaneous, footpad, intraperitoneal, and intravenous. These injections will directly influence the immune response, and harvesting lymphatic tissues and analysis of dendritic cell (DC) activation in the tissues are crucial parts of these evaluations. The extraction of mediastinal lymph nodes (mLNs) is important but quite complex because of the size and location of this organ. A stepwise procedure for harvesting the inguinal lymph node (iLN), mLN, and spleen and analyzing DC activation by flow cytometry is described.
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