Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 459, Issue 4, Pages 706-712Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2015.03.014
Keywords
Smad4; Odontoblast; Maturation; Dentin formation; Mouse
Categories
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [2013R1A2A1A01007642, 2013M3A9B20764810]
- National Research Foundation of Korea [2013R1A2A1A01007642] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ask authors/readers for more resources
The TGF-beta/BMP family plays an important role in multiple stages of tooth development. TGF-beta/BMP signaling is required for odontoblast differentiation and dentin formation; however, the precise molecular mechanisms underlying dentin formation remain unclear. To address the role of TGF-beta/BMP signaling in dentin formation, we analyzed mice in which Smad4, a key intracellular mediator of TGF-beta/BMP signaling, was subjected to tissue-specific ablation under the control of Dspp, OC, or Col1a1 promoters. Three independent Smad4 conditional knockout mice exhibited various dentin defects in the crowns and roots of their molars depending on the transactivator. In all mutant molars, crown dentin thickness was thinner than that of the control. In addition, impaired dentin was found in the cervical region and root furcation area. Although the initial differentiation of odontoblasts was normal, odontoblast polarity abruptly decreased and the expression of Col1a1, OC, and Dspp was reduced in the odontoblasts of mutant molars. In Dspp-Cre-mediated Smad4 disruption mice, primary dentin formation was slightly delayed, while secondary dentin formation was severely affected in the cervical region of the molars. These results indicate that TGF-beta/BMP signaling is required for odontoblast maturation and dentin formation in a stage- and site-dependent manner. (C) 2015 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available