Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 86, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/51559
Keywords
Medicine; Issue 86; Trauma; Nervous System; animal models; Brain trauma; in vivo multiphoton microscopy; dendrite; astrocyte; microglia; second harmonic generation
Categories
Funding
- Centre of International Mobility of Finland
- Tekes
- Finnish Graduate School of Neuroscience (FGSN)
- Academy of Finland
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Although acute brain trauma often results from head damage in different accidents and affects a substantial fraction of the population, there is no effective treatment for it yet. Limitations of currently used animal models impede understanding of the pathology mechanism. Multiphoton microscopy allows studying cells and tissues within intact animal brains longitudinally under physiological and pathological conditions. Here, we describe two models of acute brain injury studied by means of two-photon imaging of brain cell behavior under posttraumatic conditions. A selected brain region is injured with a sharp needle to produce a trauma of a controlled width and depth in the brain parenchyma. Our method uses stereotaxic prick with a syringe needle, which can be combined with simultaneous drug application. We propose that this method can be used as an advanced tool to study cellular mechanisms of pathophysiological consequences of acute trauma in mammalian brain in vivo. In this video, we combine acute brain injury with two preparations: cranial window and skull thinning. We also discuss advantages and limitations of both preparations for multisession imaging of brain regeneration after trauma.
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