Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 76, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50323
Keywords
Immunology; Issue 76; Cellular Biology; Molecular Biology; Medicine; Genetics; Biomedical Engineering; biology (general); genetics (animal and plant); immunology; life sciences; Life Sciences (General); macrophage polarization; bone marrow derived macrophage; flow cytometry; PCR; animal model
Categories
Funding
- American Heart Association [BGIA 7850037]
Ask authors/readers for more resources
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
