Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 73, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50288
Keywords
Stem Cell Biology; Issue 73; Bioengineering; Biomedical Engineering; Medicine; Molecular Biology; Cellular Biology; Anatomy; Physiology; Tissue Engineering; Cardiology; Cell Biology; Embryonic Stem Cells; ESCs; Micropatterning; Microcontact Printing; Cell Alignment; Heart Progenitors; in vitro Differentiation; Transgenic Mice; Mouse Embryonic Stem Cells; stem cells; myocardial tissue; PDMS; FACS; flow cytometry; animal model
Categories
Funding
- National Science Foundation [ECS-0335765]
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Advanced heart failure represents a major unmet clinical challenge, arising from the loss of viable and/or fully functional cardiac muscle cells. Despite optimum drug therapy, heart failure represents a leading cause of mortality and morbidity in the developed world. A major challenge in drug development is the identification of cellular assays that accurately recapitulate normal and diseased human myocardial physiology in vitro. Likewise, the major challenges in regenerative cardiac biology revolve around the identification and isolation of patient-specific cardiac progenitors in clinically relevant quantities. These cells have to then be assembled into functional tissue that resembles the native heart tissue architecture. Microcontact printing allows for the creation of precise micropatterned protein shapes that resemble structural organization of the heart, thus providing geometric cues to control cell adhesion spatially. Herein we describe our approach for the isolation of highly purified myocardial cells from pluripotent stem cells differentiating in vitro, the generation of cell growth surfaces micropatterned with extracellular matrix proteins, and the assembly of the stem cell-derived cardiac muscle cells into anisotropic myocardial tissue.
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