Journal
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
Volume -, Issue 81, Pages -Publisher
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/50802
Keywords
Medicine; Issue 81; Animal Models; Immune System Diseases; MRI; F-19 MRI; Cell Tracking; Quantification; Cell Label; In vivo Imaging
Categories
Funding
- Netherlands Institute of Regenerative Medicine (NIRM) [FES0908]
- EU FP7 program ENCITE [HEALTH-F5-2008-201842]
- TargetBraIn [HEALTH-F2-2012-279017]
- Volkswagen Foundation [I/83 443]
- Netherlands Organization for Scientific Research [VENI 700.10.409, Vidi 917.76.363]
- ERC [269019]
- Radboud University Nijmegen Medical Centre [AGIKO-2008-2-4]
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In vivo F-19 MRI allows quantitative cell tracking without the use of ionizing radiation. It is a noninvasive technique that can be applied to humans. Here, we describe a general protocol for cell labeling, imaging, and image processing. The technique is applicable to various cell types and animal models, although here we focus on a typical mouse model for tracking murine immune cells. The most important issues for cell labeling are described, as these are relevant to all models. Similarly, key imaging parameters are listed, although the details will vary depending on the MRI system and the individual setup. Finally, we include an image processing protocol for quantification. Variations for this, and other parts of the protocol, are assessed in the Discussion section. Based on the detailed procedure described here, the user will need to adapt the protocol for each specific cell type, cell label, animal model, and imaging setup. Note that the protocol can also be adapted for human use, as long as clinical restrictions are met.
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