Journal
JOURNAL OF ORAL SCIENCE
Volume 60, Issue 3, Pages 411-417Publisher
NIHON UNIV, SCHOOL DENTISTRY
DOI: 10.2334/josnusd.17-0412
Keywords
8-OHdG; oxidative stress; periapical granulomas; periapical periodontitis; SIRT1; U-937 cells
Funding
- Japan Society for the Promotion of Science [26462897]
- Sato Fund, Nihon University School of Dentistry
- Grants-in-Aid for Scientific Research [26462897] Funding Source: KAKEN
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Silent information regulator 2 homolog 1 (SIRT1) inhibits oxidative injury and has anti-inflammatory effects. SIRT1 may be involved in healing of periapical periodontitis; however, SIRTI expression in periapical periodontitis lesions has not been investigated. This study evaluated SIRTI expression and a marker of oxidative stress-8-hydroxy-2'-deoxyguanosine (8-OHdG)-in periapical granulomas. First, we used real-time polymerise chain reaction to determine whether U-937 monocytes express SIRT1. U-937 cells treated with the SIRT1 activator resveratrol exhibited the highest SIRTI mRNA level after 6-h incubation. By contrast, treating cells with the SIRT1 inhibitor sirtinol returned SIRTI expression level to that of the control. In addition, immunocytochemical analysis using cytospin specimens showed that U-937 cells co-expressed SIRTI and Ki-67. Dual-color immunofluorescence imaging showed that round cells in periapical granulomas co-expressed SIRT1 and 8-OHdG; however, neither was expressed in healthy gingival tissues. The number of 8-OHdG-expressing cells was significantly greater than the number of SIRT-expressing cells. Our findings suggest that macrophages express SIRTI and that wound healing in periapical granulomas is enhanced by a SIRT1mediated reduction in the level of oxidative stress.
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