Journal
BIOLOGY OPEN
Volume 3, Issue 6, Pages 542-552Publisher
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/bio.20148367
Keywords
Arrestin-related trafficking adaptor; ART; Arn1/Any1; Amino acid uptake; Endocytosis of transporter; Ubiquitination
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Funding
- Japan Society for the Promotion of Science [23770229]
- Hyogo Science and Technology Association [24S029]
- National Institutes of Health [CA41996]
- Grants-in-Aid for Scientific Research [23770229, 25640063, 26440053] Funding Source: KAKEN
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The Tsc1-Tsc2 complex homologous to human tuberous sclerosis complex proteins governs amino acid uptake by regulating the expression and intracellular distribution of amino acid transporters in Schizosaccharomyces pombe. Here, we performed a genetic screening for molecules that are involved in amino acid uptake and found Arn1 (also known as Any1). Arn1 is homologous to ART1, an arrestin-related trafficking adaptor (ART) in Saccharomyces cerevisiae, and contains a conserved arrestin motif, a ubiquitination site, and two PY motifs. Overexpression of arn1 + confers canavanine resistance on cells, whereas its disruption causes hypersensitivity to canavanine. We also show that Arn1 regulates endocytosis of the Cat1 amino acid transporter. Furthermore, deletion of arn1 + suppresses a defect of amino acid uptake and the aberrant Cat1 localization in tsc2D. Arn1 interacts with and is ubiquitinated by the Pub1 ubiquitin ligase, which is necessary to regulate Cat1 endocytosis. Cat1 undergoes ubiquitinations on lysine residues within the N-terminus, which are mediated, in part, by Arn1 to determine Cat1 localization. Correctively, Arn1 is an ART in S. pombe and contributes to amino acid uptake through regulating Cat1 endocytosis in which Tsc2 is involved.
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