4.3 Article

Ninein is essential for the maintenance of the cortical progenitor character by anchoring the centrosome to microtubules

Journal

BIOLOGY OPEN
Volume 2, Issue 7, Pages 739-749

Publisher

COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.20135231

Keywords

Interkinetic nuclear migration; Cortical progenitor cells; Mictrotubules; Centrosome; Pax6; Ninein; Rat

Categories

Funding

  1. MEXT Japan
  2. Global COE as a Research Associate
  3. Research Fellowship of the Japan Society for the Promotion of Science for Young Scientists
  4. Grants-in-Aid for Scientific Research [12J08042] Funding Source: KAKEN

Ask authors/readers for more resources

The mammalian cerebral cortex develops from proliferative apical progenitor cells (APs) that exhibit cell cycle-dependent nuclear movement (interkinetic nuclear migration; INM), which may be important for efficient and continuous production of neurons. The Pax6 transcription factor plays a major role in INM by regulating various downstream molecules. We have previously observed abnormal INM and unstable localization of the centrosome in APs of the Pax6 homozygous mutant rat embryo. To understand the mechanisms of INM, we focused on the centrosomes of APs. One of the centrosomal proteins, ninein, is specifically localized in the centrosome of APs. We observed a dramatic downregulation of ninein in APs of the Pax6 mutant. Moreover, knockdown of ninein by RNAi induced ectopic distribution of reduced numbers of BrdU-positive (S-phase) and PH3-positive (M-phase) cells. Furthermore, time-lapsed imaging demonstrated that knockdown of ninein in vivo induced abnormal INM. Finally, we observed impaired microtubule regrowth in neural progenitors taken from Pax6 homozygous mutant rat embryos, which was recovered by via ninein overexpression. We also found that ninein knockdown enlarged the surface size area of apical endfeet of the APs. Our results suggest that ninein plays a role in the molecular machinery essential for INM by connecting microtubules to the centrosome. (C) 2013. Published by The Company of Biologists Ltd.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available