Journal
APPLICATIONS IN PLANT SCIENCES
Volume 1, Issue 2, Pages -Publisher
BOTANICAL SOC AMER INC
DOI: 10.3732/apps.1200203
Keywords
cross-transferrable; Lagerstroemia indica; Lythraceae; microsatellite; SSR-enriched library
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Funding
- Fundamental Research Funds for the Central Universities of China [JD2010-1]
- 12th Five-Year Program for Science and Technology Development of China [2012BAD01B07]
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Premise of the study: Microsatellite markers were developed and characterized to analyze genetic diversity within Lagerstroemia cultivars and related species. Methods and Results: Using simple sequence repeat (SSR)-enriched libraries, 11 species-specific polymorphic genomic SSRs were developed from L. indica 'Hong Die Fei Wu'. All primers were tested on 48 L. indica individuals from China, the United States, and France. The primers amplified four to 12 alleles per locus, including di-, tri-, and tetranucleotide repeats. Observed and expected heterozygosities ranged from 0.1875 to 0.7609 and 0.2836 to 0.8385, respectively. The primers were also highly cross-transferrable to L. subcostata, L. limii, L. fauriei, L. caudata, and L. speciosa. Conclusions: The new primers will enlarge the bank of SSRs available to genetic research of Lagerstroemia. These SSR markers will facilitate population genetics and molecular marker-assisted selection of L. indica
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