Journal
PLANT REPRODUCTION
Volume 27, Issue 4, Pages 169-180Publisher
SPRINGER
DOI: 10.1007/s00497-014-0249-8
Keywords
MLO; Pollen hydration; Calmodulin; Rice
Categories
Funding
- Next-Generation BioGreen 21 Program, Rural Development Administration, Republic of Korea [PJ008215]
- Basic Research Promotion Fund, Republic of Korea [NRF-2007-0093862]
- Kyung Hee University [20130214]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology [2013R1A6A3A01026391]
- National Research Foundation of Korea [2013R1A6A3A01026391] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Rural Development Administration (RDA), Republic of Korea [PJ008215012014] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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MLO mediates pollen hydration. Hydration is the first step in pollen germination. However, the process is not well understood. OsMLO12 is highly expressed in mature pollen grains; plants containing alleles caused by transfer DNA insertions do not produce homozygous progeny. Reciprocal crosses between wild-type and OsMLO12/osmlo12 plants showed that the mutant alleles were not transmitted through the male gametophyte. Microscopic observations revealed that, although mutant grains became mature pollen with three nuclei, they did not germinate in vitro or in vivo due to a failure in hydration. The OsMLO12 protein has seven transmembrane motifs, with an N-terminal extracellular region and a C-terminal cytosolic region. We demonstrated that the C-terminal region mediates a calcium-dependent interaction with calmodulin. Our findings suggest that pollen hydration is regulated by MLO12, possibly through an interaction with calmodulin in the cytosol.
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