Journal
NUCLEUS
Volume 4, Issue 1, Pages 53-60Publisher
TAYLOR & FRANCIS INC
DOI: 10.4161/nucl.23384
Keywords
embryonic stem cell; nucleus; lamin A/C; lamina
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Funding
- Genentech Foundation
- George A. and Marjorie H. Anderson Fellowship
- DAAD
- NIH, National Institute of General Medical Sciences [GM42694]
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The pluripotent nature of embryonic stem cells (ESC) is associated with a dynamic open chromatin state and an irregular nuclear shape. It has been postulated that the absence of lamin A/C contributes to these features. However, we show that mouse ESCs express low, yet readily detectable, amounts of lamin A/C at both the RNA and protein levels. Full-length transcripts of both isoforms were readily detected by q-PCR and deep RNA sequencing. Additionally, protein expression was validated in multiple primary and established ESC lines by immunoblotting using several independent antibodies. Immunofluorescence labeling showed localization of lamin A/C at the nuclear periphery of all Oct4/Nanog double-positive ESC lines examined, as well as in the inner cell mass of blastocysts. Our results demonstrate ESCs do express low levels of lamin A/C, thus models linking pluripotency and nuclear dynamics with the absence of lamin A/C need to be revisited.
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