Journal
NANOSCALE RESEARCH LETTERS
Volume 8, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1186/1556-276X-8-72
Keywords
Vaccines; Gold nanoparticles; ELISPOTs; Immunotherapy; Dendritic cells; Self-assembled monolayer
Funding
- Cell and Gene Therapy Center
- Cancer Prevention Research Institute of Texas (CPRIT)
- Department of Defense Congressionally Directed Medical Research Program [USAMRAA W81XWH-07-1-0428]
- Ruth L. Kirschstein National Research Service Awards [F30CA165686]
- Medical Scientist Training Program at Baylor College of Medicine
Ask authors/readers for more resources
Nanocarriers have been explored to improve the delivery of tumor antigens to dendritic cells (DCs). Gold nanoparticles are attractive nanocarriers because they are inert, non-toxic, and can be readily endocytosed by DCs. Here, we designed novel gold-based nanovaccines (AuNVs) using a simple self-assembling bottom-up conjugation method to generate high-peptide density delivery and effective immune responses with limited toxicity. AuNVs were synthesized using a self-assembling conjugation method and optimized using DC-to-splenocyte interferon-gamma enzyme-linked immunosorbent spot assays. The AuNV design has shown successful peptide conjugation with approximately 90% yield while remaining smaller than 80 nm in diameter. DCs uptake AuNVs with minimal toxicity and are able to process the vaccine peptides on the particles to stimulate cytotoxic T lymphocytes (CTLs). These high-peptide density AuNVs can stimulate CTLs better than free peptides and have great potential as carriers for various vaccine types.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available