Journal
NANOSCALE RESEARCH LETTERS
Volume 7, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1186/1556-276X-7-443
Keywords
Porous Si; DNA immobilization; Enzyme; Nanostructure; Biosensor
Funding
- NEVET program
- Marie Curie Reintegration Grant (NANOPACK)
- Miriam and Aaron Gutwirth Excellence Scholarship Award
- Weizmann-Technion-KIT Start up grant
- DFG-CFN Excellence Initiative Project [A5.7]
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Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of horseradish peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-P,rot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-directed immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized horseradish peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild dehybridization conditions.
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