Journal
NANOSCALE RESEARCH LETTERS
Volume 7, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1186/1556-276X-7-385
Keywords
Porous silicon; Solid-phase DNA synthesis; Microparticles
Funding
- Flinders University
- Australian Research Council
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We describe the preparation of several types of porous silicon (pSi) microparticles as supports for the solid-phase synthesis of oligonucleotides. The first of these supports facilitates oligonucleotide release from the nanostructured support during the oligonucleotide deprotection step, while the second type of support is able to withstand the cleavage and deprotection of the oligonucleotides post synthesis and subsequently dissolve at physiological conditions (pH = 7.4, 37A degrees C), slowly releasing the oligonucleotides. Our approach involves the fabrication of pSi microparticles and their functionalisation via hydrosilylation reactions to generate a dimethoxytrityl-protected alcohol on the pSi surface as an initiation point for the synthesis of short oligonucleotides.
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