Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 2, Issue 14, Pages 2013-2019Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3tb21675f
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Funding
- National Basic Research Program of China (973 Program) [2013CB834701]
- Research Grants Council of Hong Kong [604711, 604913, HKUST2/CRF/10, N_HKUST620/11]
- University Grants Committee of Hong Kong [AoE/P-03/08]
- Guangdong Innovative Research Team Program [201101C0105067115]
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Lipid droplets (LDs) are subcellular organelles for energy storage and lipid metabolism regulation. Here we report an aggregation-induced emission-active fluorogen, TPE-AmAl, for specific LD imaging. TPE-AmAl is cell-permeable: upon entering the live cells, the dye molecules can selectively accumulate in the LDs and turn on the fluorescence. TPE-AmAl possesses twisted intramolecular charge transfer properties as well: the emission colour in the hydrophobic LDs is blue-shifted by >100 nm than that in aqueous buffers. Compared with the commercial lipid droplet dye, TPE-AmAl demonstrates the advantages of low background, short staining time, high selectivity, excellent biocompatibility, and good photostability. The utilization of TPE-AmAl for LD staining in green algae is also demonstrated, indicating their potential application in the high-throughput screening of high-value microalgae as a preferential biofuel source.
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