Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 2, Issue 21, Pages 3292-3298Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3tb21699c
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21273153]
- Beijing Natural Science Foundation [2132008]
Ask authors/readers for more resources
Redox probe modified reduced graphene oxide was fabricated through directly reducing graphene oxide using redox probes (toluidine blue (TB) or amino-functionalized ferrocene (NH2-Fc)). Self-assembling gold nanoparticles to fabricate redox probe modified reduced graphene oxide-Au nanocomposites via electrostatic interaction was then carried out. A sandwich-type electrochemical immunosensor, for simultaneous multianalyte detection (carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP) as model analytes), was designed. Redox probe modified reduced graphene oxide-Au nanocomposites are used as immunosensing probes and an ionic liquid functionalized reduced graphene oxide-Au (IL-rGO-Au) nanocomposite is used as an immunosensing substrate. The resulting immunosensor exhibited high selectivity and sensitivity during the simultaneous determination of CEA and AFP in a single run. The linear ranges were from 0.01 to 100 ng mL(-1) for both CEA and AFP and the detection limits reached 0.003 ng mL(-1) for CEA and 0.002 ng mL(-1) for AFP. Importantly, the proposed immunosensor was evaluated for the analysis of clinical serum samples, producing a good correlation with the data determined by the ELISA. This indicates that the immunosensor provides a possible application for the simultaneous multianalyte determination of CEA and AFP in clinical diagnostics.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available