Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 2, Issue 30, Pages 4943-4956Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3tb21665a
Keywords
-
Categories
Funding
- Portuguese Foundation for Science and Technology (FCT) [SFRH/BD/71395/2010]
- Ministerio de Economia y Competividad (MINECO) [BES2009-024735]
- European Union [REGPOT-CT2012-316331-POLARIS]
- FEDER through the Competitive Factors Operation Program -COMPETE
- National funds through FCT [PTDC/FIS/ 115048/2009, PTDC/CTM-BIO/1814/2012]
- MINECO [SAF2011-22771]
- Luso-Spanish Integrated Actions [E122/12, PRI-AIBPT-2011-1211]
- Operational Human Potential Program (POPH) developed under the scope of the National Strategic Reference Framework (QREN) from the European Social Fund (FSE)
- Fundação para a Ciência e a Tecnologia [PTDC/FIS/115048/2009, PTDC/CTM-BIO/1814/2012, SFRH/BD/71395/2010] Funding Source: FCT
Ask authors/readers for more resources
Polymeric particles acting as sources of biological cues to promote tissue regeneration are currently an interesting topic in bone tissue engineering research. In this study, microspheres of dextran-methacrylate (dextran-MA) and gamma-cyclodextrins (gamma-CD) for the delivery of osteogenic agents were prepared by means of photopolymerization on biomimetic superhydrophobic surfaces. The effects of the incorporation of the gamma-CD units as free entities or as structural monomers (acrylamidomethyl-gamma-cyclodextrin, gamma-CD-NMA) on dexamethasone loading and release performance were evaluated in detail in order to achieve osteogenic differentiation of human stem cells. The copolymerization of dextran-MA with gamma-CD-NMA improved the loading capacity of the particles and also provided a sustained release of dexamethasone for several days. The biological studies revealed that such microspheres were cytocompatible and capable of inducing the differentiation of human adipose-derived stem cells (hASCs) to osteoblasts, as determined from an increase of alkaline phosphatase (ALP) activity between days 3 and 7. Such results were also confirmed using ALP staining. Therefore, immobilization of gamma-CDs onto the dextran-MA network may be particularly useful for the development of cytocompatible implantable spherical biomaterials for bone tissue engineering purposes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available