Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 2, Issue 27, Pages 4272-4279Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4tb00488d
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Funding
- National Science Fund for Distinguished Young Scholars [21125418]
- National Natural Science Foundation of China [21174098, 21204055, 21334004]
- Natural Science Foundation of the Jiangsu Higher Education Institutions of China [13KJA430006]
- Project of Scientific and Technologic Infrastructure of Suzhou [SZS201207]
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Controlling the interface of biomaterials that take advantage of the natural fibrinolytic or clot-dissolving capacity of the body is attractive for preventing clot formation on an implanted biomaterial. Here, we engineer the interface of a biopolymer electrospun fiber mat with a serine protease of the tissue plasminogen activator (t-PA), aiming to simulate fibrinolytic functions of the body. The method is based on the one-step electrospinning aqueous solution of poly(vinyl alcohol) (PVA) and lysine ligand-modified PVA (PVA-Lys), in which the epsilon-amino and carboxyl groups of the lysine ligands were free. These electrospun mats showed good resistance to non-specific protein adsorption of fibrinogen and excellent biocompatibility with L929 cells using the MTT assay. A highly specific tethering of t-PA was facilitated by the lysine-functionalized surface through molecular recognition of t-PA to the lysine ligands. Moreover, the t-PA anchorage to the PVA/PVA-Lys mats can be easily released by plasminogen displacement when exposed to plasma, and can efficiently lyse the formed-clot in an in vitro plasma assay. In particular, the quantities of t-PA tethered on the mats could easily be regulated by simply varying the blend ratio of PVA and PVA-Lys in the electrospinning process. Collectively, considering the advantages of simplicity, controllability and biocompatibility, this approach is expected to be useful for the construction of a biointerface for blood-contacting devices.
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