Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 1, Issue 1, Pages 43-51Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2tb00203e
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Funding
- National Natural Science Foundation of China [50973082]
- National Science and Technology Major Project of China [2012AA022603]
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Monodisperse magnetic nanoparticles (MNPs) were prepared through an organic phase process, and the obtained MNPs were capped with poly[2-(2-methoxyethoxy) ethyl methacrylate]-b-poly[2-(dimethylamino) ethyl methacrylate] synthesized by surface-initiated atom transfer radical polymerization (ATRP). The MNPs-polymer brushes exhibited both superparamagnetic and thermoresponsive behaviors, and could condense plasmid DNA into nanocomplexes with a size of 100-120 nm at appropriate complexing ratios. Enhanced gene expression in COS-7 cells and HepG-2 cells was achieved under a magnetic field and variable temperature conditions due to magnetic force-facilitated internalization of nanocomplexes, and temporary cooling-triggered intracellular gene unpacking. Amazingly, combining magnetic field and temperature dual stimuli contributed to a 50-100- and 25-45-fold increase of the transfection efficiency in HepG-2 cells compared to conventional protocol and PEI25k, respectively.
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