Journal
JOURNAL OF MATERIALS CHEMISTRY B
Volume 1, Issue 5, Pages 654-660Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2tb00027j
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Funding
- NSFC [30900345]
- Programs Foundation of Ministry of Education of China [20090101120155]
- Startup Foundation of Ministry of Education for Returned Scholars [J20101127]
- Foundation of Zhejiang Provincial Department of Education [N20100475]
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In this study, proton-sponge coated quantum dots were prepared by using amphipol PMAL, grafted with polyethylenimine (PEI) as an encapsulation polymer. The QD-PMAL-PEI nanoparticles showed low cytotoxicity and superior gene silencing efficiency in serum-containing medium against junctional adhesion molecule-2 (JAM-2), which is over-expressed in glioma cells. Confocal microscopic analysis showed efficient siRNA intracellular release. In particular, QD-mediated JAM-2 knockdown was reported for the first time to facilitate inhibition of glioma cell migration. Furthermore, the Notch pathway served as the target for the JAM-2 gene function, confirmed by downregulation of its downstream genes HES1 and HES5. The unique proton-sponge coated QDs can serve as multifunctional siRNA carriers for efficient gene silencing and real-time intracellular imaging, and provide a base for design of novel efficient siRNA delivery carriers with high biocompatibility.
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