Journal
JOURNAL OF THE AMERICAN HEART ASSOCIATION
Volume 3, Issue 2, Pages -Publisher
WILEY
DOI: 10.1161/JAHA.113.000434
Keywords
apoptosis; carbonic anhydrase; diabetes mellitus; hypertrophy; microRNA
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Funding
- Italian Ministry of Education, University and Research (MIUR) FIRB-Futuro-in-Ricerca [RBFR081CCS, RBFR12I3KA]
- Italian Ministry of Health [Ricerca Finalizzata GR-2008-1142673, GR-2010-2318945]
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Background-Diabetes mellitus (DM) has multifactorial detrimental effects on myocardial tissue. Recently, carbonic anhydrases (CAs) have been shown to play a major role in diabetic microangiopathy but their role in the diabetic cardiomyopathy is still unknown. Methods and Results-We obtained left ventricular samples from patients with DM type 2 (DM-T2) and nondiabetic (NDM) patients with postinfarct heart failure who were undergoing surgical coronary revascularization. Myocardial levels of CA-I and CA-II were 6- and 11-fold higher, respectively, in DM-T2 versus NDM patients. Elevated CA-I expression was mainly localized in the cardiac interstitium and endothelial cells. CA-I induced by high glucose levels hampers endothelial cell permeability and determines endothelial cell apoptosis in vitro. Accordingly, capillary density was significantly lower in the DM-T2 myocardial samples (mean +/- SE = 2152 +/- 146 versus 4545 +/- 211/mm(2)). On the other hand, CA-II was mainly upregulated in cardiomyocytes. The latter was associated with sodium-hydrogen exchanger-1 hyperphosphorylation, exaggerated myocyte hypertrophy (cross-sectional area 565 +/- 34 versus 412 +/- 27 mu m(2)), and apoptotic death (830 +/- 54 versus 470 +/- 34 per 10(6) myocytes) in DM-T2 versus NDM patients. CA-II is activated by high glucose levels and directly induces cardiomyocyte hypertrophy and death in vitro, which are prevented by sodium-hydrogen exchanger-1 inhibition. CA-II was shown to be a direct target for repression by microRNA-23b, which was downregulated in myocardial samples from DM-T2 patients. MicroRNA-23b is regulated by p38 mitogen-activated protein kinase, and it modulates high-glucose CA-II-dependent effects on cardiomyocyte survival in vitro. Conclusions-Myocardial CA activation is significantly elevated in human diabetic ischemic cardiomyopathy. These data may open new avenues for targeted treatment of diabetic heart failure.
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