Journal
GENOME BIOLOGY
Volume 13, Issue 2, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/gb-2012-13-2-r13
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Funding
- Wellcome Trust Technology Development grant [WT088151MA]
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The accuracy of base calls produced by Illumina sequencers is adversely affected by several processes, with laser cross-talk and cluster phasing being prominent. We introduce an explicit statistical model of the sequencing process that generalizes current models of phasing and cross-talk and forms the basis of a base calling method which improves on the best existing base callers, especially when comparing the number of error-free reads. The novel algorithms implemented in All Your Base (AYB) are comparable in speed to other competitive base-calling methods, do not require training data and are designed to be robust to gross errors, producing sensible results where other techniques struggle. AYB is available at http://www.ebi.ac.uk/goldman- srv/AYB/.
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