Journal
GENOME BIOLOGY
Volume 12, Issue 3, Pages -Publisher
BMC
DOI: 10.1186/gb-2011-12-3-r22
Keywords
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Funding
- NSF
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG006102] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM083873] Funding Source: NIH RePORTER
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The biochemistry of RNA-Seq library preparation results in cDNA fragments that are not uniformly distributed within the transcripts they represent. This non-uniformity must be accounted for when estimating expression levels, and we show how to perform the needed corrections using a likelihood based approach. We find improvements in expression estimates as measured by correlation with independently performed qRT-PCR and show that correction of bias leads to improved replicability of results across libraries and sequencing technologies.
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