Journal
GENOME BIOLOGY
Volume 9, Issue 12, Pages -Publisher
BMC
DOI: 10.1186/gb-2008-9-12-r175
Keywords
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Funding
- Genoscope
- Institut de Genomique
- CEA and Agence Nationale de la Recherche (ANR)
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Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome.
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