Journal
ELIFE
Volume 7, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.37927
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Funding
- Agence Nationale de la Recherche [ANR-10-BLAN-1603, ANR-12-BSV6-0017]
- Fondation ARC pour la Recherche sur le Cancer [PJA20151203521]
- European Research Council [260358]
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung [31003A_146257, 31003A_166407]
- Centre National de la Recherche Scientifique Intramural CNRS fund
- European Research Council (ERC) [260358] Funding Source: European Research Council (ERC)
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV6-0017, ANR-10-BLAN-1603] Funding Source: Agence Nationale de la Recherche (ANR)
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The domestication of transposable elements has repeatedly occurred during evolution and domesticated transposases have often been implicated in programmed genome rearrangements, as remarkably illustrated in ciliates. In Paramecium, PiggyMac (Pgm), a domesticated PiggyBac transposase, carries out developmentally programmed DNA elimination, including the precise excision of tens of thousands of gene-interrupting germline Internal Eliminated Sequences (IESs). Here, we report the discovery of five groups of distant Pgm-like proteins (PgmLs), all able to interact with Pgm and essential for its nuclear localization and IES excision genome-wide. Unlike Pgm, PgmLs lack a conserved catalytic site, suggesting that they rather have an architectural function within a multi-component excision complex embedding Pgm. PgmL depletion can increase erroneous targeting of residual Pgm-mediated DNA cleavage, indicating that PgmLs contribute to accurately position the complex on IES ends. DNA rearrangements in Paramecium constitute a rare example of a biological process jointly managed by six distinct domesticated transposases.
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