Journal
ELIFE
Volume 3, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.04660
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Funding
- NIAID NIH HHS [K22 AI114810] Funding Source: Medline
- NICHD NIH HHS [P30 HD018655] Funding Source: Medline
- NIDCR NIH HHS [R01 DE018025] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007753] Funding Source: Medline
- NINDS NIH HHS [R37 NS039518, R01 NS038253, F32 NS076297, R01 NS070711, 1P01 NS072040-01, P01 NS072040, P50 NS040828] Funding Source: Medline
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The somatosensory nervous system is critical for the organism's ability to respond to mechanical, thermal, and nociceptive stimuli. Somatosensory neurons are functionally and anatomically diverse but their molecular profiles are not well-defined. Here, we used transcriptional profiling to analyze the detailed molecular signatures of dorsal root ganglion (DRG) sensory neurons. We used two mouse reporter lines and surface IB4 labeling to purify three major non-overlapping classes of neurons: 1) IB4(+)SNS-Cre/TdTomato(+), 2) IB4(-)SNS-Cre/TdTomato(+), and 3) ParvCre/TdTomato(+) cells, encompassing the majority of nociceptive, pruriceptive, and proprioceptive neurons. These neurons displayed distinct expression patterns of ion channels, transcription factors, and GPCRs. Highly parallel qRT-PCR analysis of 334 single neurons selected by membership of the three populations demonstrated further diversity, with unbiased clustering analysis identifying six distinct subgroups. These data significantly increase our knowledge of the molecular identities of known DRG populations and uncover potentially novel subsets, revealing the complexity and diversity of those neurons underlying somatosensation.
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