Journal
ELIFE
Volume 3, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.02637
Keywords
-
Categories
Funding
- National Institute of Health [GM-64844, R37-37120, CA-16359]
- Howard Hughes Medical Institute
- Helen Hay Whitney Foundation
- Helen L and Martin S Kimmel Center for Stem Cell Biology
- European Commission
- Deutsche Akademie der Naturforscher Leopoldina [LPDS 2009-5]
- Empire State Training Program in Stem Cell Research (NYSTEM) [C026880]
- National Cancer Institute
Ask authors/readers for more resources
Polycomb repressive complex-1 (PRC1) is essential for the epigenetic regulation of gene expression. SCML2 is a mammalian homolog of Drosophila SCM, a Polycomb-group protein that associates with PRC1. In this study, we show that SCML2A, an SCML2 isoform tightly associated to chromatin, contributes to PRC1 localization and also directly enforces repression of certain Polycomb target genes. SCML2A binds to PRC1 via its SPM domain and interacts with ncRNAs through a novel RNA-binding region (RBR). Targeting of SCML2A to chromatin involves the coordinated action of the MBT domains, RNA binding, and interaction with PRC1 through the SPM domain. Deletion of the RBR reduces the occupancy of SCML2A at target genes and overexpression of a mutant SCML2A lacking the RBR causes defects in PRC1 recruitment. These observations point to a role for ncRNAs in regulating SCML2 function and suggest that SCML2 participates in the epigenetic control of transcription directly and in cooperation with PRC1.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available