Journal
ELIFE
Volume 3, Issue -, Pages -Publisher
eLIFE SCIENCES PUBL LTD
DOI: 10.7554/eLife.02104
Keywords
Histone acetyltransferase; MSL (Male-Specific Lethal); NSL (Non-specific lethal); MOF (Male absent On the First); KAT8; H4K16ac; transcriptional activators; mouse; pluripotency; bivalent genes
Categories
Funding
- ARC
- CNRS
- INSERM
- Strasbourg University
- ANR [ANR-09-BLAN-0266, ANR-09-BLAN-0052]
- Agence Nationale de la Recherche (ANR) [ANR-09-BLAN-0266, ANR-09-BLAN-0052] Funding Source: Agence Nationale de la Recherche (ANR)
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The histone acetyltransferase (HAT) Mof is essential for mouse embryonic stem cells (mESC) pluripotency and early development. Mof is the enzymatic subunit of two different HAT complexes, MSL and NSL. The individual contribution of MSL and NSL to transcription regulation in mESCs is not well understood. Our genome-wide analysis show that i) MSL and NSL bind to specific and common sets of expressed genes, ii) NSL binds exclusively at promoters, iii) while MSL binds in gene bodies. Nsl1 regulates proliferation and cellular homeostasis of mESCs. MSL is the main HAT acetylating H4K16 in mESCs, is enriched at many mESC-specific and bivalent genes. MSL is important to keep a subset of bivalent genes silent in mESCs, while developmental genes require MSL for expression during differentiation. Thus, NSL and MSL HAT complexes differentially regulate specific sets of expressed genes in mESCs and during differentiation.
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