Journal
ADVANCED SCIENCE
Volume 2, Issue 10, Pages -Publisher
WILEY
DOI: 10.1002/advs.201500107
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Funding
- National Basic Research Program of China [2012CB933301]
- National Natural Science Foundation of China [51473078, 21171098, 21401108]
- Program for New Century Excellent Talents in University [NCET-12-0740]
- Ministry of Education of China [IRT1148]
- Synergistic Innovation Center for Organic Electronics and Information Displays, Natural Science Foundation of Jiangsu Province of China [BK20130038, BK20140866, BM2012010]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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Oxygen plays a crucial role in many biological processes. Accurate monitoring of oxygen level is important for diagnosis and treatment of diseases. Autofluorescence is an unavoidable interference in luminescent bioimaging, so that an amount of research work has been devoted to reducing background autofluorescence. Herein, a phosphorescent iridium(III) complex-modified nanoprobe is developed, which can monitor oxygen concentration and also reduce autofluorescence under both downconversion and upconversion channels. The nanoprobe is designed based on the mesoporous silica coated lanthanide-doped upconversion nanoparticles, which contains oxygen-sensitive iridium(III) complex in the outer silica shell. To image intracellular hypoxia without the interferences of autofluorescence, time-resolved luminescent imaging technology and near-infrared light excitation, both of which can reduce autofluorescence effectively, are adopted in this work. Moreover, gradient O-2 concentration can be detected clearly through confocal microscopy luminescence intensity imaging, phosphorescence lifetime imaging microscopy, and time-gated imaging, which is meaningful to oxygen sensing in tissues with nonuniform oxygen distribution.
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