Journal
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
Volume 71, Issue -, Pages 622-642Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X15008304
Keywords
cryocrystallography; cryoprotectant; flash-cooling; crystal mounting; annealing; automounter; high pressure
Funding
- NIGMS
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Cryocrystallography is an indispensable technique that is routinely used for single-crystal X-ray diffraction data collection at temperatures near 100 K, where radiation damage is mitigated. Modern procedures and tools to cryoprotect and rapidly cool macromolecular crystals with a significant solvent fraction to below the glass-transition phase of water are reviewed. Reagents and methods to help prevent the stresses that damage crystals when flash-cooling are described. A method of using isopentane to assess whether cryogenic temperatures have been preserved when dismounting screened crystals is also presented.
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