Journal
BIOMED RESEARCH INTERNATIONAL
Volume 2013, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2013/783540
Keywords
-
Funding
- Calcutta School of Tropical Medicine, Kolkata, India
Ask authors/readers for more resources
Cephalosporin resistance, particularly due to bla(TEM) encoded beta-lactamases, among Enterobacteriaceae is, though, an increasing public health problem in India; their circulating genetic variants remain unknown. The present study deals with determination of bla(TEM) variants among 134 pathogenic Enterobacteriaceae of Indian origin. Their resistance profile against 3rd generation cephalosporins was determined. The presence of bla(TEM) variants among the bacterial plasmids was characterized by PCR followed by sequencing. Intergenic relations among the variants was determined by phylogenetic analysis bla(TEM) protein were modeled by Modeller9v5 and verified. The catalytic pockets were characterized, and their interaction with cephalosporins was analyzed using AutoDock tools. More than 87% of isolates showed cephalosporin resistance with ESBL production among 57.8% of Escherichia coli and 50.6% of klebsiella pneumoniae bla(TEM-1) (84.21%), bla(TEM-1) like (3.94%), bla(TEM-33) (3.94%), bla(TEM-116) (3.94%), bla(TEM-169) (3.94%), and bla(TEM-190) (7.89%) were detected in 76 isolates. Four variants, namely, bla(TEM-1)like, bla(TEM-33), bla(TEM-169), and bla(TEM-190), coexisted in 3 isolates. The largest catalytic pocket of bla(TEM-33) explained its expanded activity towards beta-lactam-beta-lactamase inhibitor combinations. Molecular docking indicated differential resistance pattern of bla(TEM) variants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available