Journal
BIOMATERIALS SCIENCE
Volume 2, Issue 8, Pages 1073-1079Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3bm60284b
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Funding
- 973 National Basic Research Program of China [2010CB732403]
- Research Fund for the National Science Foundation of Fujian Province [2011J06003]
- Doctoral Program of Higher Education of China [20103514120003]
- National Natural Science Foundation of China [21075019, 41176079]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1116]
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A new electrochemical immunosensor was designed for the determination of carcinoembryonic antigen (CEA) with sensitivity enhanced by using nanogold poly(o-phenylenediamine)-manganese dioxide organic-inorganic hybrid nanostructures (GNPM) as nanotags and peroxidase mimics. Initially, mesoporous poly(o-phenylenediamine)-manganese dioxide (PPD-MnO2) hollow microspheres were synthesized by an inorganic/organic interfacial polymerization technique. Then gold nanoparticles were assembled onto the surface of PPD-MnO2, which were used for the labelling of the anti-CEA detection antibody (pAb(2)). The prepared GNPM nanotags were characterized using transmission electron microscopy (TEM), field emission scanning electron microscopy (FESEM), UV-vis absorption spectroscopy, N-2 adsorption-desorption isotherm measurements and Fourier transform infrared spectroscopy (FTIR). The assay was carried out with a sandwich-type immunoassay format in pH 5.5 acetic acid-buffered saline solution containing 2.5 mmol L-1 H2O2. Experimental results indicated that the electrochemical immunosensor exhibited a wide dynamic range from 0.01 to 80 ng mL(-1) towards the target CEA with a detection limit (LOD) of 6.0 pg mL(-1). The immunosensor also displayed a good stability and acceptable reproducibility and selectivity. In addition, the methodology was evaluated by assaying 10 clinical serum samples, providing a good relationship between the electrochemical immunosensor and the commercialized electrochemiluminescent (ECL) method for determination of CEA.
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