Journal
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
Volume 22, Issue 4, Pages E1-E7Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/PAI.0b013e31829928e7
Keywords
virtual slide; whole-slide imaging; indirect immunofluorescence
Funding
- Regione Lombardia [DDG 6716]
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Whole-slide images (WSI) have acquired a stable place in diagnostic histopathology and immunohistochemistry. Immunofluorescence (IF) techniques hold a limited and selective role in diagnostics (eg, renal and cutaneous pathology) and so far remain excluded from the digital pathology evolution, with notable exceptions, such as quantitative immunopathology. We explored the ability of a commercial fluorescent slide scanner to provide 4-color IF WSI from routinely processed tissues. With minor modifications and a careful match between filters and fluorochromes, we show that 4-color IF WSI can be obtained from routine material with negligible autofluorescence, good sensitivity, and diagnostic power.
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