Journal
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Volume 5, Issue -, Pages 7-15Publisher
ELSEVIER
DOI: 10.1016/j.algal.2014.04.002
Keywords
Diatoms; Silica; Lipids; Biodiesel; Algae; Sodium bicarbonate
Categories
Funding
- Environmental and Biofilm Mass Spectrometry Facility at Montana State University (MSU) through the Defense University Research Instrumentation Program (DURIP) [W911NF0510255]
- MSU Thermal Biology Institute from the NASA Exobiology Program [NAG5-8807]
- U.S. Department of Energy: Office of Biomass Programs [DE-FG36-08G018161]
- Office of Energy Efficiency and Renewable Energy (EERE) Biomass Program [DE-EE0003136]
- National Science Foundation [CHE-1230632]
- NSF-MRI Program
- M.J. Murdock Charitable Trust
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1230710] Funding Source: National Science Foundation
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [1230632, 1230609] Funding Source: National Science Foundation
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Algal biofuels represent a renewable, potentially viable, solution to mitigate transportation fuel demands. A novel diatom strain, RGd-1, isolated from Yellowstone National Park, produces high concentrations of lipids that can be converted to biodiesel. To increase the cell concentration and determine optimal conditions for growth, RGd-1 was grown without added Si, in the presence of four Si concentrations within the soluble range (0.5-2 mM), and one above the soluble range (2.5 mM). Medium Si concentrations and intracellular triacylglycerol (TAG) content were monitored daily by inductively coupled plasma mass spectrometry and Nile Red fluorescence, respectively (end-point TAG values were measured using gas chromatography). Si depletion with or without combined nitrate (NO3-) limitation was shown to induce TAG accumulation. Additionally, the effects of sodium bicarbonate (NaHCO3) supplementation were examined on cultures grown using two NO3- concentrations (2.94 and 1 mM NO3-), which also resulted in increased TAG accumulation. It was determined that utilizing a combination of two independent physiological stresses and HCO3- supplementation resulted in the highest total and per cell TAG accumulation. (C) 2014 Elsevier B.V. All rights reserved.
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