Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 1, Issue 9, Pages 740-746Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.5b00236
Keywords
fibroblast growth factor 2 (FGF-2); bio-orthogonal immobilization; genetic codon expansion; decoration; proliferation
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Funding
- BMBF (Federal Ministry of Education and Science) [13N13454]
- FET Open FP7 European project MANAQA (Magnetic Nano Actuators for Quantitative Analysis) [296679]
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Presentation of therapeutic proteins on material surfaces is challenged by random immobilization chemistries through lysine or cysteine residues, typically leading to heterogeneous product outcome. Pharmaceutical quality standards warrant a controlled process ideally through site specific conjugation. Therefore, we deployed genetic codon expansion to engineer a propargyl-L-lysine (Plk)-modified FGF-2 analogue, enabling site-specific copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Site-specific decoration of Plk-FGF-2 to particles sparked cell proliferation of human osteosarcoma cells in a spatially controlled manner around the decorated carrier, rendering this approach instrumental for the future design of quality-improved bioinstructive scaffold outcome.
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