Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 1, Issue 6, Pages 431-439Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.5b00051
Keywords
hydrogel; nerve tissue engineering peptide; RGD peptide; self-assembly; stem cell
Categories
Funding
- Biotechnology and Biological Sciences Research Council [H01716X]
- European Research Council [StG243261, ADG340764]
- Royal Society [UF051616]
- Medical Research Council [G1100623]
- Engineering and Physical Sciences Research Council (Bristol Chemical Synthesis Centre for Doctoral Training) [EP/G036764/1]
- BBSRC [BB/L01386X/1, BB/H01716X/1] Funding Source: UKRI
- EPSRC [EP/K03927X/1] Funding Source: UKRI
- MRC [G1100623] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/H01716X/1, BB/L01386X/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [1114262, EP/K03927X/1] Funding Source: researchfish
- Medical Research Council [G1100623] Funding Source: researchfish
- National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) [NC/C014103/1] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0513-10089] Funding Source: researchfish
- The British Council [GII112] Funding Source: researchfish
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Trauma to the central and peripheral nervous systems often lead to serious morbidity. Current surgical methods for repairing or replacing such damage have limitations. Tissue engineering offers a potential alternative. Here we show that functionalized alpha-helical-peptide hydrogels can be used to induce attachment, migration, proliferation and differentiation of murine embryonic neural stem cells (NSCs). Specifically, compared with undecorated gels, those functionalized with Arg-Gly-Asp-Ser (RGDS) peptides increase the proliferative activity of NSCs; promote their directional migration; induce differentiation, with increased expression of microtubule-associated protein-2, and a low expression of glial fibrillary acidic protein; and lead to the formation of larger neurospheres. Electrophysiological measurements from NSCs grown in RGDS-decorated gels indicate developmental progress toward mature neuron-like behavior. Our data indicate that these functional peptide hydrogels may go some way toward overcoming the limitations of current approaches to nerve-tissue repair.
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