Journal
TOXINS
Volume 5, Issue 12, Pages 2671-2685Publisher
MDPI
DOI: 10.3390/toxins5122671
Keywords
ochratoxin A; glucuronidation; metabolic pathway; rat liver microsomes; Orbitrap
Categories
Funding
- National Basic Research Program of China [2013CB127801]
- Shanghai Technical Standards Project [12DZ0502801]
- Shanghai Science Foundation for Youths [12ZR1448900]
- Chinese-Belgian Joint Project of BELSPO, Belgium [BL/02/C58]
- MOST, China [2012DFG31840]
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Ochratoxin A (OTA), one of the most toxic mycotoxins, can contaminate a wide range of food and feedstuff. To date, the data on its conjugates via glucuronidation request clarification and consolidation. In the present study, the combined approaches of ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS), UHPLC-Orbitrap-high resolution mass spectrometry (HRMS) and liquid chromatography-multiple stage mass spectrometry (LC-MSn) were utilized to investigate the metabolic profile of OTA in rat liver microsomes. Three conjugated products of OTA corresponding to amino-, phenol- and acyl-glucuronides were identified, and the related structures were confirmed by hydrolysis with -glucuronidase. Moreover, OTA methyl ester, OT and OT-glucuronide were also found in the reaction solution. Based on these results, an in vitro metabolic pathway of OTA has been proposed for the first time.
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