Journal
TOXINS
Volume 2, Issue 1, Pages 24-53Publisher
MDPI AG
DOI: 10.3390/toxins2010024
Keywords
botulinum neurotoxin; detection; endopeptidase; botulism; mouse lethality assay; ELISA; lateral flow test; mass spectrometry; FRET; immuno-PCR
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Funding
- National Institute of Health (NIH) [AI082190]
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [U01AI082190] Funding Source: NIH RePORTER
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Sensitive and rapid detection of botulinum neurotoxins (BoNTs), the most poisonous substances known to date, is essential for studies of medical applications of BoNTs and detection of poisoned food, as well as for response to potential bioterrorist threats. Currently, the most common method of BoNT detection is the mouse bioassay. While this assay is sensitive, it is slow, quite expensive, has limited throughput and requires sacrificing animals. Herein, we discuss and compare recently developed alternative in vitro detection methods and assess their ability to supplement or replace the mouse bioassay in the analysis of complex matrix samples.
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