Journal
STEM CELLS TRANSLATIONAL MEDICINE
Volume 3, Issue 12, Pages 1410-1417Publisher
WILEY
DOI: 10.5966/sctm.2014-0087
Keywords
Induced pluripotent stem cells; Synthetic coating; Stem cells; Regenerative medicine; iPSC; Differentiation; Feeder-free culture
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Funding
- NIH [DE016530]
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We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDSAH). Three independent transgene-free iPSC lines derived in these conditions demonstrated continuous self-renewal, genomic stability, and pluripotency in vitro and in vivo after up to 9 months of continuous in vitro culture on PMEDSAH-grafted plates. Together, these data demonstrate the strength this alternative platform offers to generate and maintain human iPSCs for regenerative medicine.
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