Journal
STEM CELL REPORTS
Volume 4, Issue 3, Pages 340-347Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2015.01.020
Keywords
-
Categories
Funding
- National Center for Advancing Translational Sciences Human Cardio-Pulmonary System on a Chip'' [UH3 TR000522]
- National Heart Lung and Blood Institute Human Pluripotent Stem Cells and Progenitor Models of Cardiac and Blood Diseases'' [U01 HL100408]
Ask authors/readers for more resources
Structural phenotyping based on classical image feature detection has been adopted to elucidate the molecular mechanisms behind genetically or pharmacologically induced changes in cell morphology. Here, we developed a set of 11 metrics to capture the increasing sarcomere organization that occurs intracellularly during striated muscle cell development. To test our metrics, we analyzed the localization of the contractile protein alpha-actinin in a variety of primary and stem-cell derived cardiomyocytes. Further, we combined these metrics with data mining algorithms to unbiasedly score the phenotypic maturity of human-induced pluripotent stem cell-derived cardiomyocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available