Journal
PROTEIN & CELL
Volume 5, Issue 9, Pages 658-672Publisher
SPRINGEROPEN
DOI: 10.1007/s13238-014-0086-4
Keywords
eukaryotic membrane proteins; recombinant expression; structural biology; integral membrane proteins (IMPs); fluorescence detected size exclusion chromatography (FSEC); protein purification and crystallization
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Funding
- National Basic Research Program (973 Program) [2011CB910501]
- National Natural Science Foundation of China [31321062-20131319400, 31125009, 91017011]
- Tsinghua-Peking Center for Life Sciences
- Howard Hughes Medical Institute
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Eukaryotic membrane proteins, many of which are key players in various biological processes, constitute more than half of the drug targets and represent important candidates for structural studies. In contrast to their physiological significance, only very limited number of eukaryotic membrane protein structures have been obtained due to the technical challenges in the generation of recombinant proteins. In this review, we examine the major recombinant expression systems for eukaryotic membrane proteins and compare their relative advantages and disadvantages. We also attempted to summarize the recent technical strategies in the advancement of eukaryotic membrane protein purification and crystallization.
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