Journal
PROTEIN & CELL
Volume 4, Issue 7, Pages 539-547Publisher
HIGHER EDUCATION PRESS
DOI: 10.1007/s13238-013-3003-3
Keywords
chrysin; osteogenesis; ERK1/2; estrogen receptor
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Funding
- National Natural Sciences Foundation of China [81173633]
- National Basic Research Program (973 Program) [2011CB707705]
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The effect of the anti-inflammatory flavonoid chrysin on osteogenesis was determined in preosteoblast MC3T3-E1 cells. Results demonstrated that chrysin could induce osteogenic differentiation in the absence of other osteogenic agents. Chrysin treatment promoted the expression of transcription factors (Runx2 and Osx) and bone formation marker genes (Col1A1, OCN, and OPN) as well as enhanced the formation of mineralized nodules. During osteogenic differentiation, chrysin preferentially activated ERK1/2, but not JNK nor the p38 MAPKs. Further experiments with inhibitors revealed the co-treatment of U0126, PD98059, or ICI182780 (a general ER antagonist) with chrysin effectively abrogated the chrysin-induced osteogenesis and ERK1/2 activation. Thus, the effect of chrysin on osteogenesis is ERK1/2-dependent and involves ER. Therefore, chrysin has the significant potential to enhance osteogenesis for osteoporosis prevention and treatment.
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