Journal
PLOS PATHOGENS
Volume 9, Issue 9, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.1003619
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Funding
- Agence Nationale de la Recherche [ANR-10-MIDI-009, CHIK-HOST-PATH2]
- Region Ile-de-France (DIM-Malinf)
- Institut Pasteur
- Fundacao para a Ciencia e a Tecnologia [SFRH/BD/60678/2009]
- National Health and Medical Research Council [461208, 637394]
- State Government of Victoria
- Australian Government
- CNRS
- Fundação para a Ciência e a Tecnologia [SFRH/BD/60678/2009] Funding Source: FCT
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Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of similar to 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.
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