Journal
PEERJ
Volume 3, Issue -, Pages -Publisher
PEERJ INC
DOI: 10.7717/peerj.1064
Keywords
De novo transcriptome assembly; Molecular markers; Chemosensory system; RNA-seq; Mygalomorphae Phylogeny
Categories
Funding
- Ministerio de Educacion y Ciencia of Spain [BFU2010-15484, CGL2013-45211, CGL2012-36863]
- Comissio Interdepartamental de Recerca i Innovacio Tecnologica of Spain [2009SGR-1287, 2014SGR1055, 2014SGR1604]
- ICREA Academia (Generalitat de Catalunya)
- IRBio fellowship (Universitat de Barcelona)
- FCA by a Juan de la Cierva postdoctoral fellowship (Spanish Ministerio de Economia y Competitividad) [JCI-2008-3456]
- Generalitat de Catalunya [2010BP-A 00438, 2010BP-B 00175]
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The funnel-web spider Macrothele calpeiana is a charismatic Mygalomorph with a great interest in basic, applied and translational research. Nevertheless, current scarcity of genomic and transcriptomic data of this species clearly limits the research in this non-model organism. To overcome this limitation, we launched the first tissue-specific enriched RNA-seq analysis in this species using a subtractive hybridization approach, with two main objectives, to characterize the specific transcriptome of the putative chemosensory appendages (palps and first pair of legs), and to provide a new set of DNA markers for further phylogenetic studies. We have characterized the set of transcripts specifically expressed in putative chemosensory tissues of this species, much of them showing features shared by chemosensory system genes. Among specific candidates, we have identified some members of the iGluR and NPC2 families. Moreover, we have demonstrated the utility of these newly generated data as molecular markers by inferring the phylogenetic position M. calpeina in the phylogenetic tree of Mygalomorphs. Our results provide novel resources for researchers interested in spider molecular biology and systematics, which can help to expand our knowledge on the evolutionary processes underlying fundamental biological questions, as species invasion or biodiversity origin and maintenance.
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