4.6 Article

Friedreich's Ataxia (GAA)(n)center dot(TTC)(n) Repeats Strongly Stimulate Mitotic Crossovers in Saccharomyces cerevisae

Journal

PLOS GENETICS
Volume 7, Issue 1, Pages -

Publisher

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1001270

Keywords

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Funding

  1. NIH [GM24110, GM52319, 1RC1ES018091, GM082950, GM60987]
  2. NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES [RC1ES018091] Funding Source: NIH RePORTER
  3. NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM052319, R01GM024110, R01GM060987, R01GM082950] Funding Source: NIH RePORTER

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Expansions of trinucleotide GAA.TTC tracts are associated with the human disease Friedreich's ataxia, and long GAA.TTC tracts elevate genome instability in yeast. We show that tracts of (GAA)(230).(TTC)(230) stimulate mitotic crossovers in yeast about 10,000-fold relative to a normal'' DNA sequence; (GAA)(n).(TTC)(n) tracts, however, do not significantly elevate meiotic recombination. Most of the mitotic crossovers are associated with a region of non-reciprocal transfer of information (gene conversion). The major class of recombination events stimulated by (GAA)(n).(TTC)(n) tracts is a tract-associated double-strand break (DSB) that occurs in unreplicated chromosomes, likely in G1 of the cell cycle. These findings indicate that (GAA)(n).(TTC)(n) tracts can be a potent source of loss of heterozygosity in yeast.

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