Journal
PLOS GENETICS
Volume 6, Issue 12, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1001254
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan [60280575]
- Core Research for Evolutionary Science and Technology (CREST) of Japan Science and Technology Agency (JST)
- Grants-in-Aid for Scientific Research [21570157] Funding Source: KAKEN
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Glycosylation plays crucial regulatory roles in various biological processes such as development, immunity, and neural functions. For example, alpha 1,3-fucosylation, the addition of a fucose moiety abundant in Drosophila neural cells, is essential for neural development, function, and behavior. However, it remains largely unknown how neural-specific a1,3-fucosylation is regulated. In the present study, we searched for genes involved in the glycosylation of a neural-specific protein using a Drosophila RNAi library. We obtained 109 genes affecting glycosylation that clustered into nine functional groups. Among them, members of the RNA regulation group were enriched by a secondary screen that identified genes specifically regulating alpha 1,3-fucosylation. Further analyses revealed that an RNA-binding protein, second mitotic wave missing (Swm), upregulates expression of the neural-specific glycosyltransferase FucTA and facilitates its mRNA export from the nucleus. This first large-scale genetic screen for glycosylation-related genes has revealed novel regulation of fucTA mRNA in neural cells.
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