Journal
PLOS GENETICS
Volume 5, Issue 2, Pages -Publisher
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1000389
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Funding
- Spanish Plan Nacional de Salud y Farmacia/CICYT [SAF2001-2262, SAF2005-08064-C04-01]
- Comunidad Autonoma de Madrid [P-BIO-0306-2006]
- Red de Terapia Celular del Instituto de Salud Carlos III (TerCel)
- Fundacion Mutua Madrilena
- Ministry of Education, and the Ministry of Health [FIS PI071023]
- Instituto de Salud Carlos III.
- Spanish Ministry of Science and Technology
- Spanish Council for Scientific Research (CSIC)
- Pfizer
- Spanish Ministry of Science and Innovation
- Pro-CNIC Foundation
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Polymerase mu (Pol mu) is an error-prone, DNA-directed DNA polymerase that participates in non-homologous end-joining (NHEJ) repair. In vivo, Pol mu deficiency results in impaired V kappa-J kappa recombination and altered somatic hypermutation and centroblast development. In Pol mu(-/-) mice, hematopoietic development was defective in several peripheral and bone marrow (BM) cell populations, with about a 40% decrease in BM cell number that affected several hematopoietic lineages. Hematopoietic progenitors were reduced both in number and in expansion potential. The observed phenotype correlates with a reduced efficiency in DNA double-strand break (DSB) repair in hematopoietic tissue. Whole-body gamma-irradiation revealed that Pol mu also plays a role in DSB repair in non-hematopoietic tissues. Our results show that Pol mu function is required for physiological hematopoietic development with an important role in maintaining early progenitor cell homeostasis and genetic stability in hematopoietic and non-hematopoietic tissues.
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